Les levures ⇒ Protocole Congelation Levure de chez wyeast

[yannick54]

Bonsoir tous le monde
pour info j ai demande a wyeast leur protocole pour congeler de la levure
voici leur reponse





Here is the protocol that you requested.

1) Mix glycerin and water in the ratio of 30 ml glycerin to 70 ml water. This will yield a 30% glycerin solution.
2) Microwave the mixture until boiling.
3) Remove to the counter, cover with saran wrap and allow to mixture cool to room temp.
4) Add equal amounts of the 30% glycerin solution and the yeast slurry to the test tube. (The amount will be dependent upon the volume of the tube.)
5) Cap the tube and shake.
6) Mark a piece of masking tape with the yeast type and label the tube.
7) Freeze

This will keep the yeast in healthy suspension for up to a year. I hope this helps, please let me know if I can be of further assistance.

Prost!

Brian Perkey, Customer Service Manager
Wyeast Laboratories, Inc.
brian@wyeastlab.com
www.wyeastlab.com
888-WYEAST-1

-----Original Message-----
Sent: Monday, March 09, 2009 12:45 AM
To: Brian Perkey
Subject: RE: Question From Wyeast Labs website for I have general question.

hello
you have a protocol with the proportions of glycerol yeast and water? How
many months will keep a ca - 20 °?
thank you


-----Message d'origine-----
De : Brian Perkey [mailto:Brian@wyeastlab.com]
Envoyé : vendredi 6 mars 2009 17:35
Objet : RE: Question From Wyeast Labs website for I have general question.


Bonjour! The only way that you can freeze yeast is to do it in a glycerin
solution. This will prevent the cell wall from rupturing during the
freeze/thaw cycle. Please let me know if I can be of further help.

À votre santé!,

Brian Perkey, Customer Service Manager
Wyeast Laboratories, Inc.
brian@wyeastlab.com
www.wyeastlab.com
888-WYEAST-1

-----Original Message-----
Sent: Friday, March 06, 2009 1:17 AM
To: Customerservice
Subject: Question From Wyeast Labs website for I have general question.


QUESTION:
hello
I'm home brewers, and I would freeze strains of yeast have your protocols
you adapt a - 20 °
thank you in advance

[Le Syphon]

Wow super utile le post!!! ..... Mais par yeast slurry à quelle étape de la fermentation fait-il appel (durant fermentation ou même a la levure de refermentation en bouteille) ?????